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| Stewien,Klaus Eberhard; Lima,Lourdes Rehder de Andrade Vaz de; Botosso,Viviane Fongaro; Oliveira,Maria Isabel de; Fagundes,Simone N.; Nogueira,Meri B.; Ragazzi,Selma Lopes Betta; Costa,Maria Tereza Zuluni da; Ejzenberg,Bernardo; Durigon,Edison Luiz. |
| A clinical and laboratory evaluation of 11 children and young adults with measleslike rash was done during the measles outbreak in the Greater São Paulo Metropolitan area at the end of 1996 and spread over the country during 1997. Measles was laboratory confirmed in 07 patients by specific IgM detection in acute serum specimens using an IgM-capture EIA, by specific IgG seroconversion in serum pairs, and by reverse transcription PCR and virus isolation in peripheral blood lymphocytes. Clinical presentations were not always classic; one of the 07 cases had received measles vaccine and corresponded to modified clinical case of measles. The 4 remaining cases were negative for measles and were diagnosed as exanthem subitum (2 cases), scarlet fever and Kawasaki... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Measles; Clinical evaluation; Laboratory investigation; Polymerase chain reaction; Measles virus isolation; Enzyme immunoassay. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400008 |
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| SILVA,Ageane Mota da; COLOMBINI,Mônica; MOURA-DA-SILVA,Ana Maria; SOUZA,Rodrigo Medeiros de; MONTEIRO,Wuelton Marcelo; BERNARDE,Paulo Sérgio. |
| ABSTRACT This study addresses the clinical and epidemiological aspects of envenoming cases resulting from snakebites treated at a hospital in Cruzeiro do Sul, in the upper Juruá River region, western Brazilian Amazonia. The specific identity of snakes that caused the envenomings was inferred (a) from the diagnosis of patient symptoms and signs upon hospital admission, (b) by enzyme immunoassay for detection of Bothrops atrox and Lachesis muta venom from serum samples taken from patients before antivenom therapy, or (c) by direct identification of the snake, when it was brought along to the hospital or photographed. There were 133 snakebites (76.2 cases per 100,000 inhabitants) registered during one year (July 2017 to June 2018). Most snakebites (88.7%)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Venomous animals; Snakes; Envenoming; Acre; Bothrops atrox; Enzyme immunoassay. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000100090 |
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| Villar,L.M.; Amado,L.A.; Gaspar,A.M.C.. |
| Hepatitis A virus (HAV) replicates relatively slowly in cell culture without a cytopathic effect, a fact that limits the use of tissue culture assays. The radioimmunofocus assay is the standard method for HAV titration, although it is labor intensive and requires the use of radioisotopes. A simple, rapid and objective infectivity assay based on an in situ enzyme immunoassay (EIA) is described here for a Brazilian cell culture-adapted HAV strain (HAF-203). The assay uses a peroxidase-labeled polyclonal antibody to fixed monolayers as an indicator of infection. EIA may be completed within 7 days using serial 5-fold dilutions of the virus, yielding a titer of 5.024 log 50% tissue culture infective dose (TCID50)/ml for HAF-203. This technique had a detection... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Hepatitis A virus; Virus titration; Enzyme immunoassay. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700011 |
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| Falci,Diego R.; Hoffmann,Elias R.; Paskulin,Diego D.; Pasqualotto,Alessandro C.. |
| Abstract The diagnosis of progressive disseminated histoplasmosis is often a challenge to clinicians, especially due to the low sensitivity and long turnaround time of the classic diagnostic methods. In recent years, studies involving a variety of non-culture-based diagnostic tests have been published in the literature. We performed a systematic review by selecting studies evaluating non-culture-based diagnostic methods for progressive disseminated histoplasmosis. We searched for articles evaluating detection of antibody, antigens, as well as DNA-based diagnostic methods. A comprehensive PUBMED, Web of Science, and Cochrane Library search was performed between the years 1956 and 2016. Case reports, review articles, non-human models and series involving... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Disseminated histoplasmosis; Non-culture; Enzyme immunoassay; Diagnostic tests. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000100007 |
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| THEAKSTON,R. D. G.; KAMIGUTI,A. S.. |
| Treatment of systemic envenoming in snakebite victims has, in the past, depended almost entirely on the individual clinician's experience in assessing the severity of envenoming. The efficacy of treatment is obviously related to the neutralising potency of the antivenom used, the route by which it is administered and the dose. The use of techniques for evaluating the efficacy of antivenoms has proved to be very useful, as an adjunct to recognised clinical observations, for a more objective evaluation of antivenom efficacy and dosage. In patients bitten by many vipers, including the Brazilian pit vipers, the reversal of the venom-induced coagulopathy provides an ideal indicator as to the efficacy of an antivenom. Likewise, the development of enzyme... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Snakebite; Therapy; Haemostasis; Enzyme immunoassay. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301998000200002 |
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